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3DISCO (stands for “3D imaging of solvent-cleared organs“) [1] is a histological method that make biological samples more transparent (so called “cleared”) by using series of organic solvents for matching the refractive index (RI) of the tissues with that of the surrounding medium.
Masson's trichrome stain of rat airway. Connective tissue is stained blue, nuclei are stained dark red/purple, and cytoplasm is stained red/pink. Mouse skin stained with Masson's trichrome stain. Masson's trichrome is a three-colour staining procedure used in histology.
The Wirtz-Conklin stain is a special technique designed for staining true endospores with the use of malachite green dye as the primary stain and safranin as the counterstain. Once stained, they do not decolourize. The addition of heat during the staining process is a huge contributing factor. [15]
The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. [1] However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods.
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.
Tissue image cytometry or tissue cytometry [1] is a method of digital histopathology and combines classical digital pathology (glass slides scanning and virtual slide generation) and computational pathology (digital analysis) into one integrated approach with solutions for all kinds of diseases, tissue and cell types as well as molecular markers and corresponding staining methods to visualize ...
Mallory's trichrome stain also called Mallory's Triple Stain is a stain utilized in histology to aid in revealing different macromolecules that make up the cell. It uses the three stains: aniline blue, acid fuchsin, and orange G. As a result, this staining technique can reveal collagen, ordinary cytoplasm, and red blood cells. It is used in ...
3Scan’s technology changes the histology workflow to automate the process of tissue sectioning and imaging. Traditionally, histology slides are prepared by embedding a tissue sample in paraffin, taking some few slices of the tissue with a hand-driven microtome, mounting the tissue slices on slides, and staining with various histology stains.