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Molecular biology is the study of the molecular underpinnings of the biological phenomena, focusing on molecular synthesis, modification, mechanisms and interactions. Biochemistry is the study of the chemical substances and vital processes occurring in living organisms .
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. [1] The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules.
Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
Biochemistry, Molecular biology: Gene knockout: Used to make one of an organism's genes inoperative ("knocked out" of the organism) Molecular biology, Genetics: Immunostaining: Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a ...
It is used in molecular biology, forensics, and medicine for the rapid identification and diagnosis of pathogenic organisms. In contrast to similar techniques such as polymerase chain reaction and ligase chain reaction , this method involves RNA transcription (via RNA polymerase ) and DNA synthesis (via reverse transcriptase ) to produce an RNA ...
Advances in molecular biology have helped show that some syndromes that were previously classed as a single disease are actually multiple subtypes with entirely different causes and treatments. Molecular diagnostics can help diagnose the subtype—for example of infections and cancers—or the genetic analysis of a disease with an inherited ...
Currently, it is a routine procedure in molecular biology or forensic analyses. This process can be done in several ways, depending on the type of the sample and the downstream application, [3] the most common methods are: mechanical, chemical and enzymatic lysis, precipitation, purification, and concentration. The specific method used to ...
Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.