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2. Van Deemter equation - Wikipedia

   en.wikipedia.org/wiki/Van_Deemter_equation

   In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column.

3. High-performance liquid chromatography - Wikipedia

   en.wikipedia.org/wiki/High-performance_liquid...

   The theory of high performance liquid chromatography-HPLC is, at its core, the same as general chromatography theory. [41] This theory has been used as the basis for system-suitability tests, as can be seen in the USP Pharmacopeia, [ 42 ] which are a set of quantitative criteria, which test the suitability of the HPLC system to the required ...

4. Chromatography column - Wikipedia

   en.wikipedia.org/wiki/Chromatography_column

   Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...

5. Chromatography - Wikipedia

   en.wikipedia.org/wiki/Chromatography

   Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid. It can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography.

6. Column chromatography - Wikipedia

   en.wikipedia.org/wiki/Column_chromatography

   Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.

7. Liquid chromatography–mass spectrometry - Wikipedia

   en.wikipedia.org/wiki/Liquid_chromatography...

   In addition to the liquid chromatography and mass spectrometry devices, an LC–MS system contains an interface that efficiently transfers the separated components from the LC column into the MS ion source. [5] [7] The interface is necessary because the LC and MS devices are fundamentally incompatible. While the mobile phase in a LC system is a ...

8. Reversed-phase chromatography - Wikipedia

   en.wikipedia.org/wiki/Reversed-phase_chromatography

   Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...

9. Monolithic HPLC column - Wikipedia

   en.wikipedia.org/wiki/Monolithic_HPLC_column

   Liquid chromatography as we know it today really got its start in 1969, when the first modern HPLC was designed and marketed as a nucleic acid analyzer. [9] Columns throughout the 1970s were unreliable, pump flow rates were inconsistent, and many biologically active compounds escaped detection by UV and fluorescence detectors. Focus on ...