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Microscope slides are often used together with a cover slip or cover glass, a smaller and thinner sheet of glass that is placed over the specimen. Slides are held in place on the microscope's stage by slide clips, slide clamps or a cross-table which is used to achieve precise, remote movement of the slide upon the microscope's stage (such as in ...
Once the specimen and water are combined on the slide, the cover glass is added. The cover glass should be placed at an angle to the slide, one edge touching the slide, and then lowered as if hinged there. If done properly, the water will force out any air as the cover glass closes over it, and no bubbles will be trapped beneath the glass ...
The glass slide can then be stained and examined. This is feasible for an initial evaluation of suspected lymphomas. An initial evaluation of a suspected lymphoma is to make a "touch prep" wherein a glass slide is lightly pressed against excised lymphoid tissue, and subsequently stained (usually H&E stain) for evaluation under light microscopy.
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.
A pair of micrographs of a cytopathology specimen showing a 3-dimensional cluster of cancerous cells (serous carcinoma) An adenocarcinoma with typical features as can be seen on cytopathology Cytopathology (from Greek κύτος , kytos , "a hollow"; [ 1 ] πάθος , pathos , "fate, harm"; and -λογία , -logia ) is a branch of pathology ...
Smear with cotton stick onto a clean glass slide; Fix w/ gentle heat or air dry; Fix w/ MeOH (Methanol) Stain w/ Giemsa, methylene blue or Wright’s stain. Microscopic examination using an oil immersion lens. (Look for multinucleated giant cells) [7]
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Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...