Search results
Results from the WOW.Com Content Network
The FMN riboswitch is able to selectively bind the FMN molecule due to several distinct nucleic acid residues, as well as some of the magnesium ions present in the overall riboswitch structure. FMN's planar isoalloxazine ring system intercalates between A48 and A85 residues on the riboswitch, thereby providing a continuous stacking alignment.
As the two strands of a double-stranded nucleic acid molecule are antiparallel, the 5′→3′ direction on the second strand corresponds to the 3′→5′ direction along the first strand. [1] [2] In general, at the most, one reading frame in a given section of a nucleic acid, is biologically relevant (open reading frame). Some viral ...
The nucleic acid notation currently in use was first formalized by the International Union of Pure and Applied Chemistry (IUPAC) in 1970. [1] This universally accepted notation uses the Roman characters G, C, A, and T, to represent the four nucleotides commonly found in deoxyribonucleic acids (DNA).
Nucleic acids present in the washed (and preferably dried) silica-nucleic acid complexes is eluted into chosen elution buffer such as TE buffer, aqua bidest, and so on. The selection of the elution buffer is co-determined by the contemplated use of the isolated nucleic acid. In this way, pure nucleic acids are isolated from the starting material.
Nucleic acid design can be used to create nucleic acid complexes with complicated secondary structures such as this four-arm junction. These four strands associate into this structure because it maximizes the number of correct base pairs, with As matched to Ts and Cs matched to Gs. Image from Mao, 2004. [5]
A typical molecular beacon structure can be divided in 4 parts: 1) loop, an 18–30 base pair region of the molecular beacon that is complementary to the target sequence; 2) stem formed by the attachment to both termini of the loop of two short (5 to 7 nucleotide residues) oligonucleotides that are complementary to each other; 3) 5' fluorophore ...
Usually 25 bases in length, they bind to complementary sequences of RNA or single-stranded DNA by standard nucleic acid base-pairing. In terms of structure, the difference between Morpholinos and DNA is that, while Morpholinos have standard nucleic acid bases, those bases are bound to methylene morpholine rings linked through phosphorodiamidate ...
These nucleotides are known as 5-(3-aminoallyl)-nucleotides since the aminoallyl group is usually attached to carbon 5 of the pyrimidine ring of uracil or cytosine. The primary amine group in the aminoallyl moiety is aliphatic and thus more reactive compared to the amine groups that are directly attached to the rings ( aromatic ) of the bases.