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Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope.
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...
Modern complete blood count analyzers can provide an automated white blood cell differential, but they have a limited ability to differentiate immature and abnormal cells, so manual examination of the blood smear is frequently indicated. [5] [6] Blood smear examination is the preferred diagnostic method for certain parasitic infections, such as ...
Leishman stain, also known as Leishman's stain, is used in microscopy for staining blood smears. It is generally used to differentiate between and identify white blood cells, malaria parasites, and trypanosomas. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin. The methanolic ...
In some cases, cells may be grown directly on a slide. For samples of loose cells (as with a blood smear or a pap smear) the sample can be directly applied to a slide. For larger pieces of tissue, thin sections (slices) are made using a microtome; these slices can then be mounted and inspected.
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Supravital stain of a smear of human blood from a patient with hemolytic anemia. The reticulocytes are the cells with the dark blue dots and curved linear structures (reticulum) in the cytoplasm. Supravital staining is a method of staining used in microscopy to examine living cells that
Cell nuclei should be crisp, blue to black on color [12] [13] and the chromatin patterns of the nucleus should be well defined. Cell cytoplasm stains blue-green and keratin stains orange in color. [13] [5] Eosin Y stains the superficial epithelial squamous cells, nucleoli, cilia, and red blood cells. [2]