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  2. Restriction fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Restriction_fragment...

    For example, the standard protocols for DNA fingerprinting involve PCR analysis of panels of more than a dozen VNTRs. RFLP is still used in marker-assisted selection. Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a technique initially developed for characterizing bacterial communities in mixed-species samples.

  3. Terminal restriction fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Terminal_restriction...

    Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based on the position of a restriction site closest to a labelled end of an amplified gene.

  4. Restriction site associated DNA markers - Wikipedia

    en.wikipedia.org/wiki/Restriction_site...

    There are other restriction site marker techniques, like RFLP or amplified fragment length polymorphism (AFLP), which use fragment length polymorphism caused by different restriction sites, for the distinction of genetic polymorphism. The use of the flanking DNA-sequences in RAD tag techniques is referred as reduced-representation method. [2]

  5. Forensic DNA analysis - Wikipedia

    en.wikipedia.org/wiki/Forensic_DNA_analysis

    Developed in 1991, [10] DQ alpha testing was the first forensic DNA technique that utilized the polymerase chain reaction. [11] This technique allowed for the use of far fewer cells than RFLP analysis making it more useful for crime scenes that did not have the large amounts of DNA material that was previously required. [12]

  6. Community fingerprinting - Wikipedia

    en.wikipedia.org/wiki/Community_Fingerprinting

    Terminal restriction fragment length polymorphism (T-RFLP) is a method that uses fluorescently-labeled DNA fragments to produce a community fingerprint. [2] [7] This section presents a brief explanation of T-RFLP in the specific context of community fingerprinting. For a more detailed explanation, refer to the T-RFLP article.

  7. DNA paternity testing - Wikipedia

    en.wikipedia.org/wiki/DNA_paternity_testing

    The current techniques for paternity testing are using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Paternity testing can now also be performed while the woman is still pregnant from a blood draw. [1] [2] DNA testing is currently the most advanced and accurate technology to determine parentage.

  8. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  9. Restriction digest - Wikipedia

    en.wikipedia.org/wiki/Restriction_digest

    Restriction digest is most commonly used as part of the process of the molecular cloning of DNA fragment into a vector (such as a cloning vector or an expression vector).The vector typically contains a multiple cloning site where many restriction site may be found, and a foreign piece of DNA may be inserted into the vector by first cutting the restriction sites in the vector as well the DNA ...

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