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Toluidine blue stain in a vasculitic peripheral neuropathy. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components. [2] It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images.
Hyaline cartilage coloured with the toluidine blue, in wich you can observe a strong metachromasia of the ground substance. View through optical microscope, 40x magnification. Metachromasia (var. metachromasy ) is a characteristic change in the color of staining carried out in biological tissues , exhibited by certain dyes when they bind to ...
The smear is first treated with chloroform to remove fats . Smear applied with Alberts stain which contains cationic dyes such as toluidine blue and malachite green. Toluidine blue preferentially stains granules while malachite green stains cytoplasm. The granules show the typical monochromatism nature, this is used to demonstrate granules
Alcians blue carries Phthalocyanine one of the most highly colored chromophores yet known with a molar extinction of 120,000 i.e. Alcian blue is detectable at half the molar concentration of popular dyes like toluidine blue, tryarylmethanes (e.g. pararosaniline and the analogous Schiff bases used in PAS stain, crystal violet in Gram stain) etc.
Staining with toluidine blue, silver stain, periodic acid-Schiff stain, or an immunofluorescence assay shows the characteristic cysts. [13] The cysts resemble crushed ping-pong balls and are present in aggregates of two to eight (and not to be confused with Histoplasma or Cryptococcus, which typically do not form aggregates of spores or cells ...
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Positive histologic stains that aid in the diagnosis of conditions of or affecting the human integumentary system Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis ...
Toluidine blue stains nuclei of malignant cells blue, and is used in oral and esophageal squamous cell carcinoma. Crystal violet is absorbed into intestinal and neoplastic cells and is used to identify Barrett's esophagus and colonic neoplasms. [citation needed] Contrast stains are not absorbed but rather provide contrast by permeating between ...