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The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR reaction. [ 3 ] NetPrimer also analyzes the thermodynamically important secondary structures such as hairpins , self and cross dimers , runs and repeats. [ 4 ]
Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA during heating. As the temperature is raised, the double strand begins to dissociate leading to a rise in the absorbance intensity, hyperchromicity. The temperature at which 50% of DNA is denatured is known as the melting temperature. Measurement ...
Freshwater environmental quality parameters are those chemical, physical and biological parameters that can be used to characterise a freshwater body. Because almost all water bodies are dynamic in their composition, the relevant quality parameters are typically expressed as a range of expected concentrations.
High resolution melting assays typically involve qPCR amplification followed by a melting curve collected using a fluorescent dye. Due to the sensitivity of high-resolution melting analysis, it is necessary to carefully consider PCR cycling conditions, template DNA quality, and melting curve parameters. [12]
The National Water Quality Inventory Report to Congress is a general report on water quality, providing overall information about the number of miles of streams and rivers and their aggregate condition. [65] The CWA requires states to adopt standards for each of the possible designated uses that they assign to their waters.
For pure elements or compounds, e.g. pure copper, pure water, etc. the liquidus and solidus are at the same temperature, and the term melting point may be used. There are also some mixtures which melt at a particular temperature, known as congruent melting. One example is eutectic mixture. In a eutectic system, there is particular mixing ratio ...
The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The melting point of the primer sets the upper limit on annealing temperature. At temperatures just above this point, only very specific base pairing between the primer and the template will occur.
It is fast (through use of a genome's FM-index) and can account for primer melting temperature and tolerated edit distances between primers and hit locations on the genome. VPCR [ 3 ] runs a dynamic simulation of multiplex PCR, allowing for an estimate of quantitative competition effects between multiple amplicons in one reaction.