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Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a cell membrane: Neuroscience, Electrophysiology: Microarray
The graph of the Dirac delta is usually thought of as following the whole x-axis and the positive y-axis. [5]: 174 The Dirac delta is used to model a tall narrow spike function (an impulse), and other similar abstractions such as a point charge, point mass or electron point.
The voltage clamp is an experimental method used by electrophysiologists to measure the ion currents through the membranes of excitable cells, such as neurons, while holding the membrane voltage at a set level. [1]
However, there are many numerical methods available to analyze the system. Certain properties and general behaviors, such as limit cycles, can be proven to exist. A simulation of the Hodgkin–Huxley model in phase space, in terms of voltage v(t) and potassium gating variable n(t). The closed curve is known as a limit cycle.
Pulse-chase analysis of auxin signal transduction in an Arabidopsis thaliana wildtype and an axr2-1 mutant. Wild-type and axr2-1 seedlings were labeled with 35S-methionine, and AXR2/axr2-1 protein was immunoprecipitated either immediately after the labeling period (t = 0) or following a 15-minute chase with unlabeled methionine (t = 15).
The methods in this section are primarily computational although they typically require data generated by wet lab experiments. Protein–protein docking , the prediction of protein–protein interactions based only on the three-dimensional protein structures from X-ray diffraction of protein crystals might not be satisfactory.
Almost all two-dimensional experiments have four stages: the preparation period, where a magnetization coherence is created through a set of RF pulses; the evolution period, a determined length of time during which no pulses are delivered and the nuclear spins are allowed to freely precess (rotate); the mixing period, where the coherence is ...
This example of design experiments is attributed to Harold Hotelling, building on examples from Frank Yates. [21] [22] [14] The experiments designed in this example involve combinatorial designs. [23] Weights of eight objects are measured using a pan balance and set of standard weights. Each weighing measures the weight difference between ...