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In optics, the numerical aperture (NA) of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light. By incorporating index of refraction in its definition, NA has the property that it is constant for a beam as it goes from one material to another, provided there is no ...
State of the art objectives can have a numerical aperture of up to 0.95. Because sin α 0 is always less than or equal to unity (the number "1"), the numerical aperture can never be greater than unity for an objective lens in air. If the space between the objective lens and the specimen is filled with oil however, the numerical aperture can ...
Nonimaging optics (also called anidolic optics) [1] [2] [3] is a branch of optics that is concerned with the optimal transfer of light radiation between a source and a target. . Unlike traditional imaging optics, the techniques involved do not attempt to form an image of the source; instead an optimized optical system for optimal radiative transfer from a source to a target is desi
Memorial in Jena, Germany to Ernst Karl Abbe, who approximated the diffraction limit of a microscope as = , where d is the resolvable feature size, λ is the wavelength of light, n is the index of refraction of the medium being imaged in, and θ (depicted as α in the inscription) is the half-angle subtended by the optical objective lens (representing the numerical aperture).
The aperture and angle of the light cone must be adjusted (via the size of the diaphragm) for each different objective lens with different numerical apertures. Condensers typically consist of a variable-aperture diaphragm and one or more lenses. Light from the illumination source of the microscope passes through the diaphragm and is focused by ...
The ability of a lens to resolve detail is usually determined by the quality of the lens, but is ultimately limited by diffraction.Light coming from a point source in the object diffracts through the lens aperture such that it forms a diffraction pattern in the image, which has a central spot and surrounding bright rings, separated by dark nulls; this pattern is known as an Airy pattern, and ...
The optical configuration for Fourier ptychography. Fourier ptychography is a computational imaging technique based on optical microscopy that consists in the synthesis of a wider numerical aperture from a set of full-field images acquired at various coherent illumination angles, [1] resulting in increased resolution compared to a conventional microscope.
All optical microscopes are diffraction-limited because of the wave nature of light. Current research focuses on techniques to go beyond this limit known as the Rayleigh criterion . The use of SIL can achieve spatial resolution better than the diffraction limit in air, for both far-field imaging [ 3 ] [ 4 ] and near-field imaging.