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Mass transfer coefficients can be estimated from many different theoretical equations, correlations, and analogies that are functions of material properties, intensive properties and flow regime (laminar or turbulent flow). Selection of the most applicable model is dependent on the materials and the system, or environment, being studied.
To calculate the transformation efficiency, divide the number of colonies by the number of cells plated and multiply by 100. The result will be the transformation efficiency as a percentage. For example, if you plate 1x 10 7 cells and count 1000 colonies, the transformation efficiency is: (1000/1x 10 7 ) x 100 = 0.1%
S phase (Synthesis phase) is the phase of the cell cycle in which DNA is replicated, occurring between G 1 phase and G 2 phase. [1] Since accurate duplication of the genome is critical to successful cell division, the processes that occur during S-phase are tightly regulated and widely conserved.
During adenylylation, there is a nucleophilic attack on the alpha phosphate of ATP from a catalytic lysine resulting in the production of inorganic pyrophosphate (PPi) and a covalently bound lysine-AMP intermediate in the active site of DNA ligase 1. During the AMP transfer step, the DNA ligase becomes associated with the DNA, locates a nick ...
B = diffusion coefficient of the eluting particles in the longitudinal direction, resulting in dispersion [m 2 s −1] C = Resistance to mass transfer coefficient of the analyte between mobile and stationary phase [s] u = speed [m s −1] In open tubular capillaries, the A term will be zero as the lack of packing means channeling does not occur ...
The Sherwood number (Sh) (also called the mass transfer Nusselt number) is a dimensionless number used in mass-transfer operation. It represents the ratio of the total mass transfer rate ( convection + diffusion) to the rate of diffusive mass transport, [ 1 ] and is named in honor of Thomas Kilgore Sherwood .
In biochemistry, a ligase is an enzyme that can catalyze the joining of two molecules by forming a new chemical bond.This is typically via hydrolysis of a small pendant chemical group on one of the molecules, typically resulting in the formation of new C-O, C-S, or C-N bonds.
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA).