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The most common reasons for laboratory semen analysis in humans are as part of a couple's infertility investigation and after a vasectomy to verify that the procedure was successful. [4] It is also commonly used for testing human donors for sperm donation, and for animals semen analysis is commonly used in stud farming and farm animal breeding.
Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA fragmentation. [1] First described by Evenson in 1980, the assay is a flow cytometric test that detects the vulnerability of sperm DNA to acid-induced denaturation DNA in situ. [2]
The resulting spermatozoa are now mature but lack motility. The mature spermatozoa are released from the protective Sertoli cells into the lumen of the seminiferous tubule in a process called spermiation. The non-motile spermatozoa are transported to the epididymis in testicular fluid secreted by the Sertoli cells with the aid of peristaltic ...
Capacitation is the penultimate [1] step in the maturation of mammalian spermatozoa and is required to render them competent to fertilize an oocyte. [2] This step is a biochemical event; the sperm move normally and look mature prior to capacitation.
In normal semen samples, centrifugation causes no more DNA damage to spermatozoa than a direct swim-up technique. [2] Washed sperm is concentrated in Ham's F-10 media without L-glutamine, warmed to 37 °C (99 °F). [3] A chemical known as a cryoprotectant is added to the sperm to aid the freezing and thawing process. [4]
Necrospermia (or necrozoospermia) is a condition in which there is a low percentage of live and a very high percentage of immotile spermatozoa in semen. [1]Necrospermia is usually confused with asthenozoospermia, which is the inability of the sperm to move even when alive.
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Several hours later (usually 2), the woman is examined by the physician. The mucus is aspirated from cervical canal and spread on a glass slide. Smear from posterior fornix is used as control. 10-50 motile sperms per high power field are considered normal. Rotatory or shaky motion of sperms indicates presence of antispermal antibody.