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For each plant, an overview (upper row; scale bar corresponds to 1 mm) and a close-up (bottom row; scale bar equals 0.5 mm) are shown. A: Haploid wild-type moss plant completely covered with leafy gametophores and close-up of wild-type leaf. B–E: Different mutants. [1] A knockout moss is a kind of genetically modified moss.
Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]
The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
The number of clones that constitute a genomic library depends on (1) the size of the genome in question and (2) the insert size tolerated by the particular cloning vector system. For most practical purposes, the tissue source of the genomic DNA is unimportant because each cell of the body contains virtually identical DNA (with some exceptions).
Ames test procedure. The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. More formally, it is a biological assay to assess the mutagenic potential of chemical compounds. [1]
Saturation mutagenesis is commonly achieved by site-directed mutagenesis PCR with a randomised codon in the primers (e.g. SeSaM) [2] or by artificial gene synthesis, with a mixture of synthesis nucleotides used at the codons to be randomised. [3] Different degenerate codons can be used to encode sets of amino acids. [1]
4437 17686 Ensembl ENSG00000113318 ENSMUSG00000014850 UniProt P20585 P13705 RefSeq (mRNA) NM_002439 NM_010829 NM_001311120 RefSeq (protein) NP_002430 NP_001298049 NP_034959 Location (UCSC) Chr 5: 80.65 – 80.88 Mb Chr 13: 92.35 – 92.49 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse DNA mismatch repair protein, MutS Homolog 3 (MSH3) is a human homologue of the bacterial mismatch ...
Therefore, genome-wide analysis is feasible if transposons are positioned throughout the genome in a mutant collection. [ 5 ] Transposon sequencing requires the creation of a transposon insertion library, which will contain a group of mutants that collectively have transposon insertions in all non-essential genes.