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Oil Red O (Solvent Red 27, Sudan Red 5B, C.I. 26125, C 26 H 24 N 4 O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a red powder with an absorbance maximum at 518 nanometers. [1]
Lipids are stained with fat soluble dyes like Sudan black. On application of Sudan black-B dyes move into lipids and are retained there while cytoplasm is counter stained with safranin. To detect the presence of lipids in cell wall, cell membrane or fat globules (PHB in cytoplasm) Lipid granules: Deep blue, Cytoplasm: Light pink 12
Solvent Black 3 is an azo dye. [1] It is a non-fluorescent, relatively thermostable lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596–605 nm and melting ...
Sudan IV (C 24 H 20 N 4 O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520(357) nm.
Sudan III is a dye used for Sudan staining. Similar dyes include Oil Red O, Sudan IV, and Sudan Black B. They are used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of reddish brown crystals and a maximum absorption at 507(304) nm. [6]
Sudan stains and Sudan dyes are synthetic organic compounds that are used as dyes for various plastics (plastic colorants) and are also used to stain sudanophilic biological samples, usually lipids. Sudan II , Sudan III , Sudan IV , Oil Red O , and Sudan Black B are important members of this class of compounds (see images below).
Sirius Red F 3B (Direct Red 80) is an azo dye primarily used in staining methods for collagen and amyloid. [1] It has the molecular formula C 45 H 26 N 10 Na 6 O 21 S 6.. In histology, sirius red staining is used in various domains of diagnostic to observe fibrosis levels in a lot of cases of inflammation induced by cancer, vascular or metabolic pathologies.
Sudan II (Solvent Orange 7, C.I. 12140, C 18 H 16 N 2 O) [1] is a lysochrome (fat-soluble dye) azo dye used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of red powder with melting point 156–158 °C and maximum absorption at 493(420) nm. [1]