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ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest.
The cost and accessibility of ChIP-seq is a major disadvantage, which has led to the more predominant use of ChIP-chip in laboratories across the world. [2] This photo compares the efficacy of the two experimental techniques, ChIP-seq and ChIP-chip. Table 1 Advantages and disadvantages of NChIP and XChIP
The major benefits of ion semiconductor sequencing are rapid sequencing speed and low upfront and operating costs. [8] [11] This has been enabled by the avoidance of modified nucleotides and optical measurements. Because the system records natural polymerase-mediated nucleotide incorporation events, sequencing can occur in real-time.
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Sequencing by ligation (SOLiD sequencing) 50+35 or 50+50 bp: 99.9%: 1.2 to 1.4 billion: 1 to 2 weeks: $60–130: Low cost per base. Slower than other methods. Has issues sequencing palindromic sequences. [109] Nanopore Sequencing: Dependent on library preparation, not the device, so user chooses read length (up to 2,272,580 bp reported [110 ...
The traditional solid-phase array is a collection of orderly microscopic "spots", called features, each with thousands of identical and specific probes attached to a solid surface, such as glass, plastic or silicon biochip (commonly known as a genome chip, DNA chip or gene array). Thousands of these features can be placed in known locations on ...
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