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The test was initially developed in the 1930s by the French medical biochemist Pierre Duquénois (1904–1986) and was adopted in the 1950s by the United Nations as the preferred test for cannabis. The test was originally claimed to be specific to cannabis. After several modifications, it became known as the Duquenois–Levine test.
THC, the main psychoactive component of cannabis, may only be detectable in saliva and oral fluid for 2–24 hours in most cases. [8] [9] The main metabolite excreted in the urine is 11-Nor-9-carboxy-THC, also known as THC-COOH. Most cannabis drug tests yield a positive result when the concentration of THC-COOH in urine exceeds 50 ng/mL. [10]
In 2015, the first government standards for testing were proposed in Colorado's legislature, when potency and microbial testing became mandatory in the state. [11] [12] [13] Colorado cannabis testing laboratories, such as AgriScience Labs, are regulated by the Colorado Department of Revenue's Marijuana Enforcement Division and the Colorado Department of Public Health and Environment. [14]
THC (found in marijuana) may only be detectable in saliva/oral fluid for 2 to 24 hours in most cases, though in rare cases has been detected up to 28 days after consumption. [15] Cocaine: 2 to 5 days (with exceptions for heavy users who can test positive up to 4/6 weeks, and individuals with certain kidney disorders) up to 90 days
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These are achieved at lower doses, because many synthetic cannabinoids are more potent than marijuana, and users are often unaware of exactly what they are getting and how potent it is. [62] For example, Δ 9-THC has an EC 50 of 250 nM at CB 1 and 1157 nM at CB 2, whereas PB-22 has an EC 50 of 5.1 nM at CB 1 and 37 nM at CB 2. [8]
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