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A monohybrid cross is a cross between two organisms with different variations at one genetic locus of interest. [ 1 ] [ 2 ] The character(s) being studied in a monohybrid cross are governed by two or multiple variations for a single location of a gene.
The forked-line method (also known as the tree method and the branching system) can also solve dihybrid and multi-hybrid crosses. A problem is converted to a series of monohybrid crosses, and the results are combined in a tree. However, a tree produces the same result as a Punnett square in less time and with more clarity.
[5] Family Boidae. Hybrids between Columbian boa and yellow anaconda. Genus Corallus. Hybrid between emerald tree boa and Amazon tree boa. Family Colubridae. A fertile cross between a king snake and a corn snake. One example is a cross between a California kingsnake and a corn snake called the "jungle corn snake." [6] [7] Genus Lampropeltis
A number of full-sib families, each produced by making crosses between the two plants from the base population are evaluated in replicated trials. A part of each full-sib family is saved for recombination. Based on evaluation the remnant seed of selected full-sib families is used to recombine the best families.
Mendel found support for this law in his dihybrid cross experiments. In his monohybrid crosses, an idealized 3:1 ratio between dominant and recessive phenotypes resulted. In dihybrid crosses, however, he found a 9:3:3:1 ratios. This shows that each of the two alleles is inherited independently from the other, with a 3:1 phenotypic ratio for each.
Termed a dihybrid cross or “two-gene test cross”, this experiment was grounded in the principle of segregation. When conducting a dihybrid test cross, two dominant phenotypic characteristics are selected and crossed with parents displaying double recessive traits. The phenotypic characteristics of the F1 generation are then analyzed.
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