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MALDI TOF/TOF mass spectrometers are used to reveal amino acid sequence of peptides using post-source decay or high energy collision-induced dissociation (further use see mass spectrometry). MALDI-TOF have been used to characterise post-translational modifications. For example, it has been widely applied to study protein methylation and ...
The original MALDESI design was implemented using common organic matrices, similar to those used in MALDI, along with a UV laser. The current MALDESI source employs endogenous water or a thin layer of exogenously deposited ice as the energy-absorbing matrix where O-H symmetric and asymmetric stretching bonds are resonantly excited by a mid-IR ...
A typical workflow of a peptide mass fingerprinting experiment. Peptide mass fingerprinting (PMF), also known as protein fingerprinting, is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. [1]
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It is a variation of matrix-assisted laser desorption/ionization (MALDI). [ 1 ] [ 2 ] In MALDI, the sample is mixed with a matrix material and applied to a metal plate before irradiation by a laser, [ 3 ] whereas in SELDI, proteins of interest in a sample become bound to a surface before MS analysis.
MALDI mass spectrometry imaging (MALDI-MSI) is the use of matrix-assisted laser desorption ionization as a mass spectrometry imaging [2] technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is recorded. [3]
Delayed extraction with laser desorption ionization. Top: the laser fires at t=0 forming a fast "red" ion and slow "blue" ion of the same m/z, Middle: the red ion takes the lead since it has a greater velocity (and kinetic energy) but since it is farther from the acceleration plate at voltage +V, it gains less energy than the blue ion.
A time-of-flight mass spectrometer (TOFMS) consists of a mass analyzer and a detector. An ion source (either pulsed or continuous) is used for lab-related TOF experiments, but not needed for TOF analyzers used in space, where the sun or planetary ionospheres provide the ions. The TOF mass analyzer can be a linear flight tube or a reflectron.