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The Ziehl-Neelsen stain, also known as the acid-fast stain, is a bacteriological staining technique used in cytopathology and microbiology to identify acid-fast bacteria under microscopy, particularly members of the Mycobacterium genus.
Ziehl–Neelsen stain (classic and modified bleach types) [5]; Kinyoun stain; For color blind people (or in backgrounds where detecting red bacteria is difficult), Victoria blue can be substituted for carbol fuchsin and picric acid can be used as the counter stain instead of methylene blue, and the rest of the Kinyoun technique can be used.
Franz Ziehl introduced the carbol fuchsin stain for the tubercle bacillus in 1882. With pathologist Friedrich Neelsen (1854–1898), he developed the Ziehl–Neelsen stain , [ 1 ] also known as the acid-fast stain, which is used to identify acid-fast bacteria .
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
It is a component of Ziehl–Neelsen stain, a differential stain. [ 2 ] [ 3 ] Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol.
English: This is a diagram of the basic steps of a Ziehl-Neelsen (Acid Fast) staining procedure File:Basic steps of acid fast staining procedure.svg is a vector version of this file. It should be used in place of this PDF file when not inferior.
Ziehl–Neelsen stain: Leprosy [nb 1] Footnotes. a b See also. List of conditions associated with café au lait macules; List of contact allergens; List of cutaneous ...
Histological sections were stained either with Haematoxylin-Eosin (HE) (A, C–E), Ziehl-Neelsen (counterstain methylenblue) (ZN) (B) .. A: Punch biopsy with large necrotic areas, fat cell ghosts and oedema but relatively intact epidermis and dermis. B: a band of extracellular AFBs is present in a deep layer of the necrotic subcutis."