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FLIP is often used and is closely associated with Fluorescence recovery after photobleaching (FRAP). The major difference between these two microscopy techniques is that FRAP involves the study of a cell’s ability to recover after a single photobleaching event whereas FLIP involves the study of how the loss of fluorescence spreads throughout ...
The split gene theory is a theory of the origin of introns, long non-coding sequences in eukaryotic genes between the exons. [1] [2] [3] The theory holds that the randomness of primordial DNA sequences would only permit small (< 600bp) open reading frames (ORFs), and that important intron structures and regulatory sequences are derived from stop codons.
This figure depicts how Floxing is used in scientific research for spatial and temporal control of gene expression. In genetic engineering, floxing refers to the insertion of a DNA sequence (which is then said to be floxed) between two LoxP sequences, creating an artificial gene cassette which can then be conditionally deleted (knocked out), translocated, or inverted in a process called Cre ...
An open reading frame (ORF) is a reading frame that has the potential to be transcribed into RNA and translated into protein. It requires a continuous sequence of DNA which may include a start codon, through a subsequent region which has a length that is a multiple of 3 nucleotides, to a stop codon in the same reading frame.
An example of a chassis is a vehicle frame, the underpart of a motor vehicle, on which the body is mounted; if the running gear such as wheels and transmission, and sometimes even the driver's seat, are included, then the assembly is described as a rolling chassis.
A container chassis, also called intermodal chassis or skeletal trailer, is a type of semi-trailer designed to securely carry an intermodal container. Chassis are used by truckers to deliver containers between ports , railyards, container depots, and shipper facilities, [ 1 ] : 2–3 and are thus a key part of the intermodal supply chain .
The ORF Finder (Open Reading Frame Finder) [16] is a graphical analysis tool which finds all open reading frames of a selectable minimum size in a user's sequence or in a sequence already in the database. This tool identifies all open reading frames using the standard or alternative genetic codes.
Fluid mosaic model of a cell membrane. The fluid mosaic model explains various characteristics regarding the structure of functional cell membranes.According to this biological model, there is a lipid bilayer (two molecules thick layer consisting primarily of amphipathic phospholipids) in which protein molecules are embedded.