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Bladder cancer is most common in wealthier regions of the world, where exposure to certain carcinogens is highest. It is also common in places where schistosome infection is common, such as North Africa. [47] Bladder cancer is much more common in men than women; around 1.1% of men and 0.27% of women develop bladder cancer. [2]
FISH, on the other hand, does not require living cells and can be quantified automatically, a computer counts the fluorescent dots present. However, a trained technologist is required to distinguish subtle differences in banding patterns on bent and twisted metaphase chromosomes. FISH can be incorporated into Lab-on-a-chip microfluidic device ...
Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, [4] whereas CISH probes are labelled with biotin or digoxigenin [5] and can be detected using a bright-field microscope after other treatment steps have ...
The test commonly checks for infection, inflammatory disease of the urinary tract, cancer, or precancerous conditions. It can be part of a broader urinalysis. If a cancerous condition is detected, other tests and procedures are usually recommended to diagnose cancers, including bladder cancer, ureteral cancer and cancer of the urethra.
Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...
Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software.
The bladder is an organ that stores urine until it is released from the body. Several conditions can affect the bladder including urinary tract infections, bladder stones, bladder cancer, overactive bladder, cystitis, interstitial cystitis, abscess, fistula, and urinary incontinence. [4] [5] [1]
Ureter cancer rarely causes problems in the early stages, but as the cancer progresses, there are often side effects. [5] Symptoms of ureteral cancer may include "blood in the urine (); diminished urine stream and straining to void (caused by urethral stricture); frequent urination and increased nighttime urination (); hardening of tissue in the perineum, labia, or penis; itching; incontinence ...
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