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Gramene [17] is an online web database resource for plant comparative genomics and pathway analysis based on Ensembl technology. Plant Genome DataBase Japan [18] (PGDBj) is a website that contains information related to genomes of model and crop plants from databases. It has three main components: ortholog db, DNA marker and linkage map db, and ...
Many plants are well known for natural cloning ability, including blueberry plants, Hazel trees, the Pando trees, [10] [11] the Kentucky coffeetree, Myrica, and the American sweetgum. It also occurs accidentally in the case of identical twins, which are formed when a fertilized egg splits, creating two or more embryos that carry identical DNA.
A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. [1] The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.
P1 has the ability to copy a bacteria's host genome and integrate that DNA information into other bacteria hosts, also known as generalized transduction. [2] Later on, P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s. It is capable of Cre-Lox recombination.
The choice of vector for molecular cloning depends on the choice of host organism, the size of the DNA to be cloned, and whether and how the foreign DNA is to be expressed. [7] The DNA segments can be combined by using a variety of methods, such as restriction enzyme/ligase cloning or Gibson assembly. [citation needed]
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Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...
Once a clone from a genomic library is sequenced, the sequence can be used to screen the library for other clones containing inserts which overlap with the sequenced clone. Any new overlapping clones can then be sequenced forming a contig. This technique, called chromosome walking, can be exploited to sequence entire chromosomes. [2]