Search results
Results from the WOW.Com Content Network
The zebrafish (Danio rerio) is a species of freshwater ray-finned fish belonging to the family Danionidae of the order Cypriniformes.Native to South Asia, [3] it is a popular aquarium fish, frequently sold under the trade name zebra danio [4] (and thus often called a "tropical fish" although it is both tropical and subtropical).
Zebrafish is an important vertebrate and emerged as an important model for genetics, developmental biology, chemical biology, and regeneration. In order to establish cell culture about 200-300 zebrafish Ab strain embryos at 5-10 somite stage were dechlorinated by 300 ul/mL protease treatment and washed with PBS.
The GloFish is a patented and trademarked brand of fluorescently colored genetically modified aquarium fish.They have been created from several different species of fish: zebrafish were the first GloFish available in pet stores, and recently the black tetra, tiger barb, [1] rainbow shark, Siamese fighting fish, X-ray tetra, and most recently bronze corydoras [2] have been added to the lineup.
Fish embryos go through a process called mid-blastula transition which is observed around the tenth cell division in some fish species. Once zygotic gene transcription starts, slow cell division begins and cell movements are observable. [ 4 ]
Two species of fish, zebrafish (Danio rerio) and medaka (Japanese rice fish, Oryzias latipes), are most commonly modified because they have optically clear chorions (shells), develop rapidly, the 1-cell embryo is easy to see and micro-inject with transgenic DNA, and zebrafish have the capability of regenerating their organ tissues. [9]
In seed plants, the embryo will develop one or more "seed leaves" . By the end of embryogenesis, the young plant will have all the parts necessary to begin its life. Once the embryo germinates from its seed or parent plant, it begins to produce additional organs (leaves, stems, and roots) through the process of organogenesis.
GM fish are widely used in basic research in genetics and development. Two species of fish- zebrafish and medaka, are most commonly modified, because they have optically clear chorions (membranes in the egg), rapidly develop, and the 1-cell embryo is easy to see and microinject with transgenic DNA. [105]
Up to 18% of Morpholinos appear to induce nontarget-related phenotypes including cell death in the central nervous system and somite tissues of zebrafish embryos. [43] Most of these effects are due to activation of p53-mediated apoptosis and can be suppressed by co-injection of an anti-p53 Morpholino along with the experimental Morpholino.