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  2. COVID-19 testing - Wikipedia

    en.wikipedia.org/wiki/COVID-19_testing

    COVID-19 Antigen Rapid Test Kit; the timer is provided by the user. Mucus from nose or throat in a test liquid is placed onto a COVID-19 rapid antigen diagnostic test device. COVID-19 rapid testing in Rwanda. An antigen is the part of a pathogen that elicits an immune response. Antigen tests look for antigen proteins from the viral surface.

  3. Uracil-DNA glycosylase - Wikipedia

    en.wikipedia.org/wiki/Uracil-DNA_glycosylase

    In this method the primers used in PCR are synthesized with uracil residues instead of thymine. When these primers are incorporated into PCR amplified fragments the primer sequence becomes susceptible to digestion with Uracil N Glycosylase and produce 3' protruding ends that can be annealed to an appropriately prepared vector DNA.

  4. Reverse transcription polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Reverse_transcription...

    First combine template RNA, primer, dNTP mix, and nuclease-free water in a PCR tube. Then, add an RNase inhibitor and reverse transcriptase to the PCR tube. Next, place the PCR tube into a thermal cycler for one cycle wherein annealing, extending, and inactivating of reverse transcriptase occurs.

  5. Development of COVID-19 tests - Wikipedia

    en.wikipedia.org/wiki/Development_of_COVID-19_tests

    Public Health England announced a test on the 10th, [13] using a real-time RT-PCR (RdRp gene) assay based on oral swabs. [14] The test detected the presence of any type of coronavirus, including specifically identifying SARS-CoV-2. It was rolled out to twelve laboratories across the United Kingdom on 10 February. [15]

  6. Reverse Transcription Loop-mediated Isothermal Amplification

    en.wikipedia.org/wiki/Reverse_Transcription_Loop...

    The outer primers(F3 and B3) anneal to the template strand and help the reaction to proceed. As in the case of RT-PCR , the RT-LAMP procedure starts by making DNA from the sample RNA. This conversion is made by a reverse transcriptase , an enzyme derived from retroviruses capable of making such a conversion. [ 15 ]

  7. Why you have COVID symptoms and a negative test: You ... - AOL

    www.aol.com/finance/why-covid-symptoms-negative...

    At-home COVID tests “work as well as they always have”—even on new variants like “Pirola” BA.2.86, ... you can obtain a PCR, or polymerase chain reaction, test at a doctor’s office ...

  8. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  9. What Really Causes a False Positive COVID-19 Test? Experts ...

    www.aol.com/lifestyle/false-positive-covid-19...

    The government program that mailed free COVID-19 test kits to Americans came to an end when the CDC announced the end of the public health emergency in May 2023. But an iteration is coming back ...