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  2. Two-dimensional nuclear magnetic resonance spectroscopy

    en.wikipedia.org/wiki/Two-dimensional_nuclear...

    Almost all two-dimensional experiments have four stages: the preparation period, where a magnetization coherence is created through a set of RF pulses; the evolution period, a determined length of time during which no pulses are delivered and the nuclear spins are allowed to freely precess (rotate); the mixing period, where the coherence is ...

  3. Nuclear magnetic resonance spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Nuclear_magnetic_resonance...

    Other types of two-dimensional NMR include J-spectroscopy, exchange spectroscopy (EXSY), Nuclear Overhauser effect spectroscopy (NOESY), total correlation spectroscopy (TOCSY), and heteronuclear correlation experiments, such as HSQC, HMQC, and HMBC. In correlation spectroscopy, emission is centered on the peak of an individual nucleus; if its ...

  4. Nuclear Overhauser effect - Wikipedia

    en.wikipedia.org/wiki/Nuclear_Overhauser_effect

    Professor Anil Kumar was the first to apply the two-dimensional Nuclear Overhauser Effect (2D-NOE now known as NOESY) experiment to a biomolecule, which opened the field for the determination of three-dimensional structures of biomolecules in solution by NMR spectroscopy.

  5. Nuclear magnetic resonance - Wikipedia

    en.wikipedia.org/wiki/Nuclear_magnetic_resonance

    In two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR), there will be one systematically varied time period in the sequence of pulses, which will modulate the intensity or phase of the detected signals. In 3D-NMR, two time periods will be varied independently, and in 4D-NMR, three will be varied.

  6. Nuclear magnetic resonance spectroscopy of proteins

    en.wikipedia.org/wiki/Nuclear_magnetic_resonance...

    With unlabelled protein the usual procedure is to record a set of two-dimensional homonuclear nuclear magnetic resonance experiments through correlation spectroscopy (COSY), of which several types include conventional correlation spectroscopy, total correlation spectroscopy (TOCSY) and nuclear Overhauser effect spectroscopy (NOESY). [3] [4] A ...

  7. Nuclear magnetic resonance spectroscopy of nucleic acids

    en.wikipedia.org/wiki/Nuclear_magnetic_resonance...

    Two-dimensional NMR methods are almost always used with nucleic acids. These include correlation spectroscopy (COSY) and total coherence transfer spectroscopy (TOCSY) to detect through-bond nuclear couplings, and nuclear Overhauser effect spectroscopy (NOESY) to detect couplings between nuclei that are close to each other in space.

  8. Jean Jeener - Wikipedia

    en.wikipedia.org/wiki/Jean_Jeener

    Jeener is best known for introducing two-dimensional NMR spectroscopy (2DNMR). In a lecture at the AMPERE Summer School in Basko Polje, Yugoslavia, September 1971, [6] [7] he proposed a novel technique, later known as Correlation Spectroscopy (COSY), in which the response of the nuclear spins to two radio frequency pulses is treated by a double Fourier transformation with respect to the delay ...

  9. Heteronuclear single quantum coherence spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Heteronuclear_single...

    The resulting spectrum is two-dimensional (2D) with one axis for proton (1 H) and the other for a heteronucleus (an atomic nucleus other than a proton), which is usually 13 C or 15 N. The spectrum contains a peak for each unique proton attached to the heteronucleus being considered.