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Blunt-end ligation is also reversibly inhibited by high concentration of ATP. [23] PCR usually generates blunt-ended PCR products, but note that PCR using Taq polymerase can add an extra adenine (A) to the 3' end of the PCR product. This property may be exploited in TA cloning where the ends of the PCR product can anneal to the T end of a vector.
The simplest DNA end of a double stranded molecule is called a blunt end. Blunt ends are also known as non-cohesive ends. In a blunt-ended molecule, both strands terminate in a base pair. Blunt ends are not always desired in biotechnology since when using a DNA ligase to join two molecules into one, the yield is significantly lower with blunt ...
Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950. [ 2 ]
Retrieved from "https://en.wikipedia.org/w/index.php?title=Cohesive_end&oldid=476062181"This page was last edited on 10 February 2012, at 03:38 (UTC). (UTC).
Researchers from the University of British Columbia have developed a "groundbreaking coating" that could make blood-contacting devices safer. Dr. Jayachandran Kizhakkedathu discusses the benefits.
RingMyCellphone is free up to three times per day. Its wake-up function is also free, from anywhere in the world. You can use it free up to two times a day; after that, it's $2 for up to five more ...
WASHINGTON - Businessmen Elon Musk and Vivek Ramaswamy will meet with House Republicans next week to discuss cuts to federal government spending, among other matters.. The move comes as the pair ...
Adhesive bonding is a joining technique used in the manufacture and repair of a wide range of products. Along with welding and soldering, adhesive bonding is one of the basic joining processes. In this technique, components are bonded together using adhesives. The broad range of types of adhesives available allows numerous materials to be ...