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Cosmids are plasmids that incorporate a segment of bacteriophage λ DNA that has the cohesive end site (cos) which contains elements required for packaging DNA into λ particles. Under apt origin of replication (ori), it can replicate as a plasmid.
Before site specific recombination can occur, the oligonucleotide ends must be filled. The ligation of these ends generates a replication fork at each end of the transposable element. The single strand displacement causes synthesis from the un-ligated 3' hydroxyl group to form long single stranded sections adjacent to the 5' end.
The first step in Gateway cloning is the preparation of a Gateway Entry clone. There are a few different ways to make entry clone. Gateway attB1 and attB2 sequences are added to the 5' and 3' end of a gene fragment, respectively, using gene-specific PCR primers and PCR amplification. The PCR amplification products are then mixed with a propriet
The integration of phage λ takes place at a special attachment site in the bacterial and phage genomes, called att λ. The sequence of the bacterial att site is called attB, between the gal and bio operons, and consists of the parts B-O-B', whereas the complementary sequence in the circular phage genome is called attP and consists of the parts ...
The bacteriophage P1 was first isolated by Dr. Giuseppe Bertani.In his study, he noticed that the lysogen produced abnormal non-continuous phages, and later found phage P1 was produced from the Lisbonne lysogen strain, in addition to bacteriophages P2 and P3.
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The base pair formed between the nucleotide and the template base fits nicely into a groove between the fingers and the 3’-end of the primer. [8] Two Mg 2+ ions form an octahedral coordinate network with oxygen ligand and also bring the reactive primer hydroxyl and the nucleotide α-phosphate close together, thereby lowering the entropic cost ...
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