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For the first time, scientists have peered into living cells and created videos showing how they function with unprecedented 3D detail. Using a special microscope and new lighting techniques, a ...
There's a problem in cell biology research: to study what happens inside a cell, it has to be destroyed. When scientists use a traditional microscope to observe a cell, they use stains ...
The Inner Life of the Cell is an 8.5-minute 3D computer graphics animation illustrating the molecular mechanisms that occur when a white blood cell in the blood vessels of the human body is activated by inflammation (Leukocyte extravasation).
A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. [1]
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.
First, the cell genomes are cross-linked with formaldehyde, [28] which introduces bonds that "freeze" interactions between genomic loci. Treatment of cells with 1-3% formaldehyde, for 10-30min at room temperature is most common, however, standardization for preventing high protein-DNA cross linking is necessary, as this may negatively affect ...
Most light sheet fluorescence microscopes are used to produce 3D images of the sample by moving the sample through the image plane. If the sample is larger than the field of view of the image sensor, the sample also has to be shifted laterally. An alternative approach is to move the image plane through the sample to create the image stack. [32]
3D cell culture methods have been developed to enable research into the behavior of cells in an environment that more accurately represents their interactions in-vivo.. 3D cell culturing by magnetic levitation uses biocompatible polymer-based reagents [2] to deliver magnetic nanoparticles to individual cells so that an applied magnetic driver can levitate cells off the bottom of the cell ...