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A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision , from single piece glass pipettes to more complex adjustable or ...
Micropipette in action. Piston-driven air displacement pipettes are a type of micropipette, which are tools to handle volumes of liquid in the microliter scale. They are more commonly used in biology and biochemistry, and less commonly in chemistry; the equipment is susceptible to damage from many organic solvents.
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop. After about one second, keep on pressing pipette knob to the second stop. This action will completely empty the tip from the liquid. Afterwards withdraw the tip from the liquid and release the pipette to the ready position.
Positive displacement pipettes are a type of pipette that operates via piston-driven displacement. [1] [2] Unlike an air displacement pipette, which dispenses liquid using an air cushion in the pipette tip, the piston in a positive displacement pipette makes direct contact with the sample, allowing the aspiration force to remain constant.
There are two basic types of microinjection systems. The first is called a constant flow system and the second is called a pulsed flow system.In a constant flow system, which is relatively simple and inexpensive though clumsy and outdated, a constant flow of a sample is delivered from a micropipette and the amount of the sample which is injected is determined by how long the needle remains in ...
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