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The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
A two-photon microscope is also a laser-scanning microscope, but instead of UV, blue or green laser light, a pulsed infrared laser is used for excitation. Only in the tiny focus of the laser is the intensity high enough to generate fluorescence by two-photon excitation , which means that no out-of-focus fluorescence is generated, and no pinhole ...
Refraction of light is the most commonly observed phenomenon, but other waves such as sound waves and water waves also experience refraction. How much a wave is refracted is determined by the change in wave speed and the initial direction of wave propagation relative to the direction of change in speed.
The speed of light waves in air is approximately 3.0×10 8 m/s (exactly 299,792,458 m/s in vacuum). The wavelength of visible light waves varies between 400 and 700 nm, but the term "light" is also often applied to infrared (0.7–300 μm) and ultraviolet radiation (10–400 nm).
In physics, backscatter (or backscattering) is the reflection of waves, particles, or signals back to the direction from which they came. It is usually a diffuse reflection due to scattering , as opposed to specular reflection as from a mirror , although specular backscattering can occur at normal incidence with a surface.
Reflection of light is either specular (mirror-like) or diffuse (retaining the energy, but losing the image) depending on the nature of the interface.In specular reflection the phase of the reflected waves depends on the choice of the origin of coordinates, but the relative phase between s and p (TE and TM) polarizations is fixed by the properties of the media and of the interface between them.
Light from the source is polarized in the x direction after passing through the first polarizer, but above the specimen is a polarizer (a so-called analyzer) oriented in the y direction. Therefore, no light from the source will be accepted by the analyzer, and the field will appear dark.
Fermat's principle is most familiar, however, in the case of visible light: it is the link between geometrical optics, which describes certain optical phenomena in terms of rays, and the wave theory of light, which explains the same phenomena on the hypothesis that light consists of waves.