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A shaker is a piece of laboratory equipment used to mix, blend, or agitate substances in a tube or flask by shaking them. It is mainly used in the fields of chemistry and biology . A shaker contains an oscillating board that is used to place the flasks, beakers , or test tubes.
During suspension cell culturing, shaker flasks are loaded with cells and the appropriate media before they are placed on an orbital shaker. To optimize cell culture proliferation, the revolutions per minute of the orbital shaker must be adjusted within an acceptable range depending on the cells and media used.
Rockers are often used in place of shakers when less aggressive mixing is required. Rockers are commonly used for staining and de-staining gels after electrophoresis , hybridization , [ 1 ] washing, blotting , [ 2 ] Cell culture [ 3 ] and gentle mixing.
Vortex mixers are quite commonplace in bioscience laboratories. In cell culture and microbiology laboratories they may be used to suspend cells. In a biochemical or analytical laboratory they may be used to mix the reagents of an assay or to mix an experimental sample and a dilutant.
The main components of a scanning tunneling microscope are the scanning tip, piezoelectrically controlled height (z axis) and lateral (x and y axes) scanner, and coarse sample-to-tip approach mechanism. The microscope is controlled by dedicated electronics and a computer. The system is supported on a vibration isolation system. [5]
Slater-type orbitals (STOs) or Slater-type functions (STFs) are functions used as atomic orbitals in the linear combination of atomic orbitals molecular orbital method. They are named after the physicist John C. Slater , who introduced them in 1930.
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
The Paschen–Back effect is the splitting of atomic energy levels in the presence of a strong magnetic field. This occurs when an external magnetic field is sufficiently strong to disrupt the coupling between orbital and spin angular momenta. This effect is the strong-field limit of the Zeeman effect.
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