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  2. PstI - Wikipedia

    en.wikipedia.org/wiki/PstI

    These hybrid DNA molecules can be then cleaved at the regenerated PstI sites. Its use is not limited to molecular cloning; it is also used in restriction site mapping, genotyping, Southern blotting, restriction fragment length polymorphism (RFLP) and SNP. [9] It is also an isoschizomer restriction enzyme SalPI from Streptomyces albus P. [10]

  3. 15 Most Common Puppy Health Issues and How to Spot Them - AOL

    www.aol.com/15-most-common-puppy-health...

    Worms and other internal parasites can be treated easily but are some of the most common problems seen in dogs. Some of the internal parasites that cause diarrhea and loose stools in puppies ...

  4. Cosmid - Wikipedia

    en.wikipedia.org/wiki/Cosmid

    Cosmids are predominantly plasmids with a bacterial oriV, an antibiotic selection marker and a cloning site, but they carry one, or more recently two, cos sites derived from bacteriophage lambda. Depending on the particular aim of the experiment, broad host range cosmids, shuttle cosmids or 'mammalian' cosmids (linked to SV40 oriV and mammalian ...

  5. Cloning vector - Wikipedia

    en.wikipedia.org/wiki/Cloning_vector

    Cosmids are plasmids that incorporate a segment of bacteriophage λ DNA that has the cohesive end site (cos) which contains elements required for packaging DNA into λ particles. Under apt origin of replication (ori), it can replicate as a plasmid.

  6. Lambda phage - Wikipedia

    en.wikipedia.org/wiki/Lambda_phage

    The integration of phage λ takes place at a special attachment site in the bacterial and phage genomes, called att λ. The sequence of the bacterial att site is called attB, between the gal and bio operons, and consists of the parts B-O-B', whereas the complementary sequence in the circular phage genome is called attP and consists of the parts ...

  7. 15 Foods You Should Never Share With Your Dog—No ... - AOL

    www.aol.com/15-foods-never-share-dog-224100146.html

    For dogs, xylitol can cause a dangerous drop in blood sugar and liver failure. 6. Alcohol. It might seem obvious, but even small amounts of alcohol can have serious effects on a dog’s liver and ...

  8. Today's Wordle Hint, Answer for #1257 on Wednesday, November ...

    www.aol.com/lifestyle/todays-wordle-hint-answer...

    Today's Wordle Answer for #1257 on Wednesday, November 27, 2024. Today's Wordle answer on Wednesday, November 27, 2024, is SLANG. How'd you do? Next: Catch up on other Wordle answers from this week.

  9. Φ29 DNA polymerase - Wikipedia

    en.wikipedia.org/wiki/Φ29_DNA_polymerase

    The polymerase is a monomeric protein with two distinct functional domains. Site-directed mutagenesis experiments support the proposition that this protein displays a structural and functional similarity to the Klenow fragment of the Escherichia coli Polymerase I enzyme; [3] it comprises a C-terminal polymerase domain and a spatially separated N-terminal domain with a 3'-5' exonuclease activity.