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Supercritical fluid chromatography (SFC) [1] is a form of normal phase chromatography that uses a supercritical fluid such as carbon dioxide as the mobile phase. [2] [3] It is used for the analysis and purification of low to moderate molecular weight, thermally labile molecules and can also be used for the separation of chiral compounds.
Supercritical fluid chromatography (SFC) can be used on an analytical scale, where it combines many of the advantages of high performance liquid chromatography (HPLC) and gas chromatography (GC). It can be used with non-volatile and thermally labile analytes (unlike GC) and can be used with the universal flame ionization detector (unlike HPLC ...
Supercritical fluid chromatography (SFC) Principle is very similar to that of HPLC. But SFC typically uses carbon dioxide as the mobile phase. Hence there is a need to pressurize the entire chromatographic flow path. SFC can differentiate enantiomers and enantiomer from the racemate; (+) from (-) and (±) Chiral capillary electrophoresis (CE) [45]
Photograph of a separatory funnel in a laboratory scale extraction of 2 immiscible liquids: liquids are a diethyl ether upper phase, and a lower aqueous phase. Soxhlet extractor Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix .
Carbon dioxide (CO 2) is usually pumped as a liquid, usually below 5 °C (41 °F) and a pressure of about 50 bar. The solvent is pumped as a liquid as it is then almost incompressible; if it were pumped as a supercritical fluid, much of the pump stroke would be "used up" in compressing the fluid, rather than pumping it.
Carbon dioxide pressure-temperature phase diagram This video shows the property of carbon dioxide to go into a supercritical state with increasing temperature. Supercritical carbon dioxide (s CO 2) is a fluid state of carbon dioxide where it is held at or above its critical temperature and critical pressure.
Supercritical fluid extraction is a relatively new technique for extracting fragrant compounds from a raw material, which often employs supercritical CO 2 as the extraction solvent. When carbon dioxide is put under high pressure at slightly above room temperature, a supercritical fluid forms (Under normal pressure CO 2 changes directly from a ...
Liquid CO 2 washing, like supercritical fluid CO 2 washing, relies on the high solvent power of CO 2, [4]: 275 but at lower temperatures and pressures, the latter making it simpler to implement. Because liquid CO 2 does not have the solvent power of the supercritical fluid, agitation and surfactants may be added to improve the effectiveness of ...