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The blood is mixed with a small amount of sterile water to cause hemolysis of the RBCs, yielding free hemoglobin. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes.
Acid–base extraction is a subclass of liquid–liquid extractions and involves the separation of chemical species from other acidic or basic compounds. [1] It is typically performed during the work-up step following a chemical synthesis to purify crude compounds [2] and results in the product being largely free of acidic or basic impurities.
A basic salt of an alkali metal or alkaline earth metal [2] (this includes Mg(OH) 2 (magnesium hydroxide) but excludes NH 3 ). Any base that is soluble in water and forms hydroxide ions [3] [4] or the solution of a base in water. [5] (This includes both Mg(OH) 2 and NH 3, which forms NH 4 OH.) The second subset of bases is also called an ...
Sodium hydroxide, also known as lye and caustic soda, [1] [2] is an inorganic compound with the formula NaOH. It is a white solid ionic compound consisting of sodium cations Na + and hydroxide anions OH −. Sodium hydroxide is a highly corrosive base and alkali that decomposes lipids and proteins at ambient temperatures and may cause severe ...
An example is the H 2 O (water) molecule, which can gain a proton to form the hydronium ion, H 3 O +, or lose a proton, leaving the hydroxide ion, OH −. The relative ability of a molecule to give up a proton is measured by its pK a value. A low pK a value indicates that the compound is acidic and will easily give up its proton to a base.
One advantage of this low solubility is that "many antacids were suspensions of metal hydroxides such as aluminium hydroxide and magnesium hydroxide"; [9] compounds with low solubility and the ability to stop an increase in the concentration of the hydroxide ion, preventing the harm of the tissues in the mouth, oesophagus, and stomach. [9]
An aqueous sample is treated with an equal volume of 1% strong base (sodium or potassium hydroxide) followed by a few drops of aqueous copper(II) sulfate. If the solution turns purple, it contains protein. 5–160 mg/mL can be determined. Peptides with the correct length of at least 3 amino acids are necessary for a significant, measurable ...
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