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Under the microscope, the India ink stain is used for easy visualization of the capsule in cerebral spinal fluid. [10] The particles of ink pigment do not enter the capsule that surrounds the spherical yeast cell, resulting in a zone of clearance or "halo" around the cells. This allows for quick and easy identification of C. neoformans.
The dying yeast cells are then heated to complete their breakdown, after which the husks (yeast with thick cell walls that would give poor texture) are removed. Yeast autolysates are used in Vegemite and Promite (Australia); Marmite (the United Kingdom); the unrelated Marmite (New Zealand); Vitam-R (Germany); and Cenovis (Switzerland).
Two forms of yeast cells can survive and grow: haploid and diploid. The haploid cells undergo a simple lifecycle of mitosis and growth, and under conditions of high stress will, in general, die. This is the asexual form of the fungus. The diploid cells (the preferential 'form' of yeast) similarly undergo a simple lifecycle of mitosis and growth.
The ability to switch between yeast cells and hyphal cells is an important virulence factor. Many proteins play a role in this very complex process. [ 111 ] The formation of hyphae can for example help Candida albicans to escape from macrophages in the human body. [ 112 ]
Single cell state of Yarrowia lipolytica under microscope. The cells of Y. lipolytica have over 20% fat content, placing it in the group of oleaginous yeasts. [10] Most lipids are stored as triacylglycerids (TAGs). This physiological trait makes this species especially interesting for producing lipid derivates.
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Fission yeast is easily accessible, easily grown and manipulated to make mutants, and able to be maintained at either a haploid or diploid state. S. pombe is normally a haploid cell but, when put under stressful conditions, usually nitrogen deficiency, two cells will conjugate to form a diploid that later form four spores within a tetrad ascus ...
Under the microscope Tinea versicolor is recognized by curved hyphae and round yeast forms that give it a spaghetti-and-meatball appearance. Yeast cells appear round or oval and budding forms may be seen. The KOH prep cannot identify the specific organism; the specimen can be submitted for fungal culture to identify the organism.