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In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
Since URA3 allows for both positive and negative selection, it has been developed as a genetic marker for DNA transformations and other genetic techniques in bacteria and many fungal species. It is one of the most important genetic markers in yeast genetic modification. While URA3 is a powerful selectable marker, it has a high background.
The yeast form of S. schenckii is effectively phagocytosed by cells of the innate immune system [34] and are recognized based on the sugars displayed on their surface [35] or lipids in the yeast cell membrane. [34] Although they are taken up, they are not efficiently killed.
Malassezia pachydermatis is a zoophilic yeast in the division Basidiomycota.It was first isolated in 1925 by Fred Weidman, and it was named pachydermatis (Greek for 'thick-skin') after the original sample taken from an Indian rhinoceros (Rhinocerosus unicornis) with severe exfoliative dermatitis. [2]
Kluyveromyces marxianus is an aerobic yeast capable of respiro-fermentative metabolism that consists of simultaneously generating energy from both respiration via the TCA cycle and ethanol fermentation. [3] The balance between respiration and fermentation metabolisms is strain specific. [9]
Selectable markers allow scientists to separate non-recombinant organisms (those which do not contain the selectable marker) from recombinant organisms (those which do); that is, a recombinant DNA molecule such as a plasmid expression vector is introduced into bacterial cells, and some bacteria are successfully transformed while some remain non-transformed.
Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) [1] and protein–DNA interactions [2] [3] by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.
Mannans from other yeast, for example candida albicans, have found to cross react with ASCA which suggests that other yeast may induce ASCA associated diseases. ASCA are serological markers of candida albicans infections in humans and animals. [1] Mannan-binding lectin is a lectin produced by humans. In ASCA positive Crohn's disease the serum ...