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Add 2.84 mM of HCl to shift the buffer to 7.3 mM HPO 4 2− and 4.6 mM H 2 PO 4 − for a final pH of 7.4 and a Cl − concentration of 142 mM. The pH of PBS is ~7.4. When making buffer solutions, it is good practice to always measure the pH directly using a pH meter. If necessary, pH can be adjusted using hydrochloric acid or sodium hydroxide.
McIlvaine buffer is a buffer solution composed of citric acid and disodium hydrogen phosphate, also known as citrate-phosphate buffer. It was introduced in 1921 by the United States agronomist Theodore Clinton McIlvaine (1875–1959) from West Virginia University , and it can be prepared in pH 2.2 to 8 by mixing two stock solutions.
THC, the main psychoactive component of cannabis, may only be detectable in saliva and oral fluid for 2–24 hours in most cases. [8] [9] The main metabolite excreted in the urine is 11-Nor-9-carboxy-THC, also known as THC-COOH. Most cannabis drug tests yield a positive result when the concentration of THC-COOH in urine exceeds 50 ng/mL. [10]
THC-O-phosphate is a water-soluble organophosphate ester derivative of tetrahydrocannabinol (THC), which functions as a metabolic prodrug for THC itself.It was invented in 1978 in an attempt to get around the poor water solubility of THC and make it easier to inject for the purposes of animal research into its pharmacology and mechanism of action.
RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer can be stored without vanadate at 4 °C for up to 1 year. [10] RIPA buffer releases proteins from cells as well as disrupts most weak interactions between proteins. [9] Recipe: [10] 1% (w/w) Nonidet P-40 (NP-40)
Tyrode's solution is a solution that is roughly isotonic with interstitial fluid and used in physiological experiments and tissue culture. It resembles lactated Ringer's solution, but contains magnesium, a sugar (usually glucose) as an energy source and uses bicarbonate and phosphate as a buffer instead of lactate.
A similar case reported in June 1995 in the United Kingdom.The description of that case appears to indicate the convicted manufacturer was using D. Gold's book Cannabis Alchemy as a guide. [19] THC acetate was also reported to have been found by New Zealand police in 1995, again made by acetylation of purified cannabis extracts with acetic ...
Panama red, known as Panamanian red, [1] or P.R. [2] is a pure cultivar of Cannabis sativa. It is popular amongst cannabis users of the 1960s and 1970s, and renowned for its potency. [ 3 ] [ 4 ] [ 5 ] The typically high THC levels associated with the variety are thought to be dependent on the particular cultivar, rather than the Panamanian climate.