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SSMD is the ratio of mean to the standard deviation of the difference between two groups. When the data is preprocessed using log-transformation as we normally do in HTS experiments, SSMD is the mean of log fold change divided by the standard deviation of log fold change with respect to a negative
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The commonly used dual-flashlight plot is for the difference between two groups in high-throughput experiments such as microarrays and high-throughput screening studies, in which we plot the SSMD versus average log fold-change on the y- and x-axes, respectively, for all genes or compounds (such as siRNAs or small molecules) investigated in an ...