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  2. Antibiotic sensitivity testing - Wikipedia

    en.wikipedia.org/wiki/Antibiotic_sensitivity_testing

    Antibiotic resistance tests: Bacteria are streaked on dishes with white disks, each impregnated with a different antibiotic. Clear rings, such as those on the left, show that bacteria have not grown—indicating that these bacteria are not resistant. The bacteria on the right are fully resistant to all but two of the seven antibiotics tested. [33]

  3. Phenotypic testing of mycobacteria - Wikipedia

    en.wikipedia.org/wiki/Phenotypic_testing_of_Myco...

    The test differs from that used to detect catalase in other types of bacteria by using 30% hydrogen peroxide in a strong detergent solution (10% polysorbate 80). [1] Citrate. Sole carbon source [1] Egg medium. Growth on Löwenstein–Jensen medium (LJ medium) L-Glutamate. Sole carbon and nitrogen source [1] Growth rate

  4. Analytical profile index - Wikipedia

    en.wikipedia.org/wiki/Analytical_profile_index

    The analytical profile index, or API, is a classification system for bacteria based on biochemical tests. The system was developed to accelerate the speed of identifying clinically relevant bacteria. It can only be used to identify known species from an index. [1] The data obtained are phenotypic traits.

  5. Diagnostic microbiology - Wikipedia

    en.wikipedia.org/wiki/Diagnostic_Microbiology

    The catalase test tests whether a microbe produces the enzyme catalase, which catalyzes the breakdown of hydrogen peroxide. Smearing a colony sample onto a glass slide and adding a solution of hydrogen peroxide (3% H 2 O 2) will indicate whether the enzyme is present or not. Bubbling is a positive test while nothing happening is a negative ...

  6. Lysine iron agar - Wikipedia

    en.wikipedia.org/wiki/Lysine_iron_agar

    Lysine iron agar or LIA is a differential media used to distinguish bacteria that are able to decarboxylate lysine and/or produce hydrogen sulfide from those that cannot. [1] This test is particularly useful for distinguishing different Gram-negative bacilli —especially among the Enterobacteriaceae .

  7. Obligate aerobe - Wikipedia

    en.wikipedia.org/wiki/Obligate_aerobe

    They can be found evenly spread throughout the test tube. An obligate aerobe is an organism that requires oxygen to grow. [ 1 ] Through cellular respiration , these organisms use oxygen to metabolise substances, like sugars or fats, to obtain energy.

  8. Disk diffusion test - Wikipedia

    en.wikipedia.org/wiki/Disk_diffusion_test

    The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture-based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the susceptibility ...

  9. Colonial morphology - Wikipedia

    en.wikipedia.org/wiki/Colonial_morphology

    Colonial morphology serves as the first step in the identification of microbial species from clinical samples. [10] Based on the visual appearance of the colonies, microbiologists can narrow down the list of possible organisms, allowing them to select appropriate tests to provide a definitive diagnosis.