Search results
Results from the WOW.Com Content Network
Both cause excessive urination (hence the similarity in name), but whereas diabetes insipidus is a problem with the production of antidiuretic hormone (neurogenic diabetes insipidus) or the kidneys' response to antidiuretic hormone (nephrogenic diabetes insipidus), diabetes mellitus causes polyuria via osmotic diuresis, due to the high blood ...
Diabetes insipidus (DI) is a condition characterized by large amounts of dilute urine and increased thirst. [1] The amount of urine produced can be nearly 20 liters per day. [ 1 ] Reduction of fluid has little effect on the concentration of the urine. [ 1 ]
Transient expression, more frequently referred to "transient gene expression", is the temporary expression of genes that are expressed for a short time after nucleic acid, most frequently plasmid DNA encoding an expression cassette, has been introduced into eukaryotic cells with a chemical delivery agent like calcium phosphate (CaPi) or polyethyleneimine (PEI). [1]
Gene knockdown is an experimental technique by which the expression of one or more of an organism's genes is reduced. The reduction can occur either through genetic modification or by treatment with a reagent such as a short DNA or RNA oligonucleotide that has a sequence complementary to either gene or an mRNA transcript.
A fluid or water deprivation test is a medical test [1] which can be used to determine whether the patient has diabetes insipidus as opposed to other causes of polydipsia (a condition of excessive thirst that causes an excessive intake of water). The patient is required, for a prolonged period, to forgo intake of water completely, to determine ...
Ectopic expression using these techniques is a useful tool because phenotypes induced in a tissue or cell type where are not normally expressed are easily distinguishable compared to a tissue or cell type where the gene is normally expressed. By the comparison with its basal expression, the function of a gene of interest can be identified. [3]
A cell line derived from the cabbage looper is of particular interest, as it has been developed to grow fast and without the expensive serum normally needed to boost cell growth. [ 27 ] [ 28 ] The shuttle vector is called bacmid, and gene expression is under the control of a strong promoter pPolh. [ 29 ]
Madin-Darby canine kidney (MDCK) cells are a model mammalian cell line used in biomedical research. MDCK cells are used for a wide variety of cell biology studies including cell polarity , cell-cell adhesions (termed adherens junctions ), collective cell motility, toxicity studies, [ 1 ] as well as responses to growth factors.