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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
Sodium dihydrogen phosphate Sodium hydrogen phosphate Trisodium phosphate. A sodium phosphate is a generic variety of salts of sodium (Na +) and phosphate (PO 3− 4). Phosphate also forms families or condensed anions including di-, tri-, tetra-, and polyphosphates. Most of these salts are known in both anhydrous (water-free) and hydrated forms ...
Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is an inorganic compound with the chemical formula Na 2 H P O 4. It is one of several sodium phosphates . The salt is known in anhydrous form as well as hydrates Na 2 HPO 4 · n H 2 O , where n is 2, 7, 8, and 12.
Sodium phosphates including monosodium phosphate, disodium phosphate, and trisodium phosphate are approved as food additives in the EU. They are commonly used as acidity regulators and have the collective E number E339. [11] The United States Food and Drug Administration lists sodium phosphates as generally recognized as safe. [12] [13]
Tetrasodium pyrophosphate, also called sodium pyrophosphate, tetrasodium phosphate or TSPP, is an inorganic compound with the formula Na 4 P 2 O 7. As a salt, it is a white, water-soluble solid. It is composed of pyrophosphate anion and sodium ions. Toxicity is approximately twice that of table salt when ingested orally. [3]
Monosodium phosphate (MSP), also known as monobasic sodium phosphate and sodium dihydrogen phosphate, is an inorganic compound with the chemical formula Na H 2 P O 4. It is a sodium salt of phosphoric acid. It consists of sodium cations (Na +) and dihydrogen phosphate anions (H 2 PO − 4). One of many sodium phosphates, it is a common ...
RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer can be stored without vanadate at 4 °C for up to 1 year. [10] RIPA buffer releases proteins from cells as well as disrupts most weak interactions between proteins. [9] Recipe: [10] 1% (w/w) Nonidet P-40 (NP-40)
Dibasic sodium phosphate, ethylenediamine tetra acetic acid[ sodium (EDTA), fetal bovine serum, gelatin, glutamate, monobasic potassium phosphate, monobasic sodium phosphate, monosodium L-glutamate, MRC-5 DNA and cellular protein, neomycin, phosphate, potassium chloride, sucrose: Yellow fever vaccine Egg protein, gelatin, sorbitol