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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
IPTG, a molecule similar to lactose, but with a sulfur bond that is not hydrolyzable so that E. coli does not digest it, is used to activate or "induce" the production of the new protein. Once the cells are induced, it is difficult to remove IPTG from the cells and therefore it is difficult to stop expression.
Cowlick vs. Balding: Key Differences. A cowlick differs from a bald spot in a couple key ways.. First, a cowlick is a natural, normal feature of your scalp that occurs as a result of your genes.
Cuban authorities struggled to return power to the island on Thursday morning after Hurricane Rafael knocked out the country`s electrical grid, leaving 10 million people in the dark. The grid ...
A look at where the various court cases against President-elect Donald Trump stand, and how they may — or may not — be affected by his taking the oath of office on Jan. 20.
Fortnite is an online video game and game platform developed by Epic Games and released in 2017. It is available in seven distinct game mode versions that otherwise share the same general gameplay and game engine: Fortnite Battle Royale, a free-to-play battle royale game in which up to 100 players fight to be the last person standing; Fortnite: Save the World, a cooperative hybrid tower ...
H-1B visas allow the brightest foreign workers, many of them engineers and computer scientists, to live and work in the US. Supporters say they’re vital to ensuring Silicon Valley innovation ...