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Buffer capacity rises to a local maximum at pH = pK a. The height of this peak depends on the value of pK a. Buffer capacity is negligible when the concentration [HA] of buffering agent is very small and increases with increasing concentration of the buffering agent. [3] Some authors show only this region in graphs of buffer capacity. [2]
Good sought to identify buffering compounds which met several criteria likely to be of value in biological research. p K a : Because most biological reactions take place near-neutral pH between 6 and 8, ideal buffers would have p K a values in this region to provide maximum buffering capacity there.
Bis-Tris propane wide buffering range is also useful for calibration of genetically encoded pH indicators expressed in the cytosol or mitochondria. [6] Bis-Tris propane has been used as the buffering agent in separation of full and empty capsids of recombinant adeno-associated virus vectors with anion-exchange chromatography. [7] [8]
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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
Food processing is the transformation of agricultural products into food, or of one form of food into other forms. Food processing takes many forms, from grinding grain into raw flour , home cooking , and complex industrial methods used in the making of convenience foods .
The useful buffer range for tris (pH 7–9) coincides with the physiological pH typical of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory. Tris is also used as a primary standard to standardize acid solutions for chemical analysis.
Borate buffered saline (abbreviated BBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Borate buffers have an alkaline buffering capacity in the 8–10 range. Boric acid has a pK a of 9.14 at 25 °C.