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DNA primase is an enzyme involved in the replication of DNA and is a type of RNA polymerase.Primase catalyzes the synthesis of a short RNA (or DNA in some living organisms [1]) segment called a primer complementary to a ssDNA (single-stranded DNA) template.
The E. Coli DnaG primase is a 581 residue monomeric protein with three functional domains, according to proteolysis studies. There is an N-terminal Zinc-binding domain (residues 1–110) where a zinc ion is tetrahedrally coordinated between one histidine and three cysteine residues, which plays a role in recognizing sequence specific DNA binding sites.
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Along the DNA template, primase intersperses RNA primers that DNA polymerase uses to synthesize DNA from in the 5′→3′ direction. [1] Another example of primers being used to enable DNA synthesis is reverse transcription. Reverse transcriptase is an enzyme that uses a template strand of RNA to synthesize a complementary strand of DNA.
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DNA primase small subunit is an enzyme that in humans is encoded by the PRIM1 gene. [ 5 ] The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components.
There are urban legends in the Philippines purporting supposed inventions by Filipinos. These assertions are presented as facts in some academic textbooks in history and science used by Filipino students, as well as social media, to promote Filipino exceptionalism. [61] Fluorescent lamp, said to be invented by a certain Agapito Flores.
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