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The secondary benefit of using spectrophotometric analysis for nucleic acid quantitation is the ability to determine sample purity using the 260 nm:280 nm calculation. The ratio of the absorbance at 260 and 280 nm (A 260/280) is used to assess the purity of nucleic acids.
Abundance in weight: spectroscopic nucleic acid quantitation; Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR) High-throughput relative abundance: DNA microarray; High-throughput absolute abundance: serial analysis of gene expression (SAGE) Size: gel electrophoresis
The Qubit fluorometer method is to use fluorescent dyes to determine the concentration of either nucleic acids or proteins in a sample. Specialized fluorescent dyes bind specifically to the substances of interest.
The polymerase chain reaction method is used to quantify nucleic acids by amplifying a nucleic acid molecule with the enzyme DNA polymerase. [2] Conventional PCR is based on the theory that amplification is exponential.
RiboGreen is a proprietary fluorescent dye that is used in the detection and quantification of nucleic acids, including both RNA and DNA. It is synthesized and marketed by Molecular Probes/Invitrogen (a division of Life Technologies, now part of Thermo Fisher Scientific) of Eugene, Oregon, United States. In its free form, RiboGreen exhibits ...
Nucleic acids are complex, high-molecular-weight biochemical macromolecules composed of nucleotide chains. The main article for this category is Nucleic acids . Wikimedia Commons has media related to Nucleic acids .
The original nucleic acid arrays were macro arrays approximately 9 cm × 12 cm and the first computerized image based analysis was published in 1981. [1] It was invented by Patrick O. Brown. An example of its application is in SNPs arrays for polymorphisms in cardiovascular diseases, cancer, pathogens and GWAS analysis.
Nucleic acid quantitation; Molecular interactions; Enzyme activity; Cell toxicity, proliferation, and viability; ATP quantification; Immunoassays [11] High throughput screening of compounds and targets in drug discovery (Labeled Alpha Screen on most instruments) [12] Bead-based epitope assay [13] Cellular Uptake of nanoparticles [14]