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Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol. DTT is redox reagent also known as Cleland's reagent, after W. Wallace Cleland. [2] The reagent is commonly used in its racemic form. Its name derives from the four-carbon sugar, threose.
The reduction potential of DTE is the same as for DTT, about –0.331 mV. [2] The pK a values of the thiol groups of DTE are 9.0 and 9.9, which is higher than the corresponding values for DTT (9.3 and 9.5). [2] Since reduction of disulfide bonds requires thiolate (ionized thiol), DTE is less efficient at lower pH compared to DTT. [2]
A common 1,4-dithiol is dithiothreitol (DTT), HSCH 2 CH(OH)CH(OH)CH 2 SH, sometimes called Cleland's reagent, for to reduce protein disulfide bonds. Oxidation of DTT results a stable six-membered heterocyclic ring with an internal disulfide bond. Reduction of a typical disulfide bond by DTT via two sequential thiol-disulfide exchange reactions.
2-Mercaptoethanol is often used interchangeably with dithiothreitol (DTT) or the odorless tris(2-carboxyethyl)phosphine (TCEP) in biological applications. Although 2-mercaptoethanol has a higher volatility than DTT, it is more stable: 2-mercaptoethanol's half-life is more than 100 hours at pH 6.5 and 4 hours at pH 8.5; DTT's half-life is 40 ...
The epithelial DNA in solution is removed and saved, while the sperm cell's DNA precipitates with the attached protamines. Differential extraction uses a chemical called dithiothreitol (DTT) to disrupt the sulfur bonds in the protamines in order to release its DNA. Once the DNA is detached from the protamines, it is prone to standard DNA ...
Dithiothreitol (DTT) This page was last edited on 24 March 2021, at 21:44 (UTC). Text is available under the Creative Commons Attribution-ShareAlike 4.0 License ...
On the image, the DTT is 2R-3R at the beginning and at the final, it is 2S-3R, please change the image! Talk about L-DTT and D-DTT. Usually DTT is a DL-DTT racemic mixture, but it is important to do the difference. —Preceding unsigned comment added by 132.204.84.95 (talk • contribs) Please correct the stereochemistry!
The reduction to the thiol is accomplished by the reaction with chemicals containing sulfhydryl or phosphine groups such as dithiothreitol (DTT) or tris-2-carboxyethylphosphine hydrochloride (TCEP). In course of the subsequent irreversible alkylation of the SH groups with iodoacetamide the cysteines are transformed to the stable S ...