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Leghemoglobin (also leghaemoglobin or legoglobin) is an oxygen-carrying phytoglobin found in the nitrogen-fixing root nodules of leguminous plants. It is produced by these plants in response to the roots being colonized by nitrogen-fixing bacteria, termed rhizobia, as part of the symbiotic interaction between plant and bacterium: roots not colonized by Rhizobium do not synthesise leghemoglobin.
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The nitrogen-fixing clade consists of four orders of flowering plants: Cucurbitales, Fabales, Fagales and Rosales. [a] This subgroup of the rosids encompasses 28 families of trees, shrubs, vines and herbaceous perennials and annuals. The roots of many of the species host bacteria that fix nitrogen into compounds the plants can use. [4] [5]
The majority of pre-packaged foods are required to list all ingredients, including all food additives such as color; however section B.01.010 (3)(b) of the Regulations provide food manufacturers with the choice of declaring added color(s) by either their common name or simply as "colour".
For example, nitrogen compounds comprise 40% to 50% of the dry matter of protoplasm, and it is a constituent of amino acids, the building blocks of proteins. [9] It is also an essential constituent of chlorophyll. [10] In many agricultural settings, nitrogen is the limiting nutrient for rapid growth.
The reagent does not measure only phenols, but will react with any reducing substance. It therefore measures the total reducing capacity of a sample, not just phenolic compounds. This reagent is part of the Lowry protein assay , and will also react with some nitrogen-containing compounds such as hydroxylamine and guanidine . [ 3 ]
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