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The DNA attaches to the flow cell via complementary sequences. The strand bends over and attaches to a second oligo forming a bridge. A polymerase synthesizes the reverse strand. The two strands release and straighten. Each forms a new bridge (bridge amplification). The result is a cluster of DNA forward and reverse strand clones.
A bridge-parallel amplifier topology is a hierarchical combination of the bridged and paralleled amplifier topologies, with at least four single-ended channels needed to produce one bridge-parallel channel. The two topologies complement each other in that the bridging allows for higher voltage output and the paralleling provides the current ...
Clonal Bridge Amplification Reversible Dye Terminator 2x300 0.17-2.7 15 Illumina HiSeq Clonal Bridge Amplification Reversible Dye Terminator 2x150 0.3-11 [12] 1000 [13] Illumina Genome Analyzer IIX Clonal Bridge Amplification Reversible Dye Terminator [14] [15] 2x150 2-14 95 Life Technologies SOLiD4 Clonal-emPCR Oligonucleotide 8-mer Chained ...
The technology features bridge amplification to generate clusters and reversible terminators for sequence determination. [ 52 ] [ 53 ] The technology behind these sequencing systems involves ligation of fragmented DNA to a chip, followed by primer addition and sequential fluorescent dNTP incorporation and detection.
However, other earlier patented technologies, such as that from Manteia Predictive Medicine (acquired by Solexa), which generate DNA on a solid-phase surface by bridge amplification, are generally referred to as "clusters". The terminology and distinction between 'polony' and 'cluster' have become confused recently.
Reduces the 6dB attenuation incurred by impedance matching, which helps by reducing the amount of make-up amplification required [5] and by maintaining a high signal-to-noise ratio. [6] [4] However a transformer can be used instead to match impedance and provide better signal-to-noise. And the 6dB attenuation can be easily be made up in the ...
The loop gain is a product of the very high amplifier gain and the very low bridge ratio. [26] In Hewlett's circuit, the amplifier is implemented by two vacuum tubes. The amplifier's inverting input is the cathode of tube V 1 and the non-inverting input is the control grid of tube V 2.
The data may also contain errors, caused by limitations in the DNA sequencing technique or by errors during PCR amplification. DNA sequencer manufacturers use a number of different methods to detect which DNA bases are present. The specific protocols applied in different sequencing platforms have an impact in the final data that is generated.
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