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  2. Auxotrophy - Wikipedia

    en.wikipedia.org/wiki/Auxotrophy

    In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...

  3. Saccharomyces Genome Database - Wikipedia

    en.wikipedia.org/wiki/Saccharomyces_Genome_Database

    In the peer-reviewed literature report, experimental results on function and interaction of yeast genes are extracted by high-quality manual curation and integrated within a well-developed database. The data are combined with quality high-throughput results and posted on Locus Summary pages which is a powerful query engine and rich genome browser.

  4. URA3 - Wikipedia

    en.wikipedia.org/wiki/URA3

    URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase (ODCase) , which is an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides (a component of RNA ).

  5. Kluyveromyces lactis - Wikipedia

    en.wikipedia.org/wiki/Kluyveromyces_lactis

    In the 1990s, few genes were known and analysed by scientists until the first genomic analysis was performed by a team of the Pasteur Institute of Paris. [2] The genome Kluyveromyces lactis was explored by sequencing 588 short tags from two random genomic libraries (random sequenced tags, or RSTs). 296 K. lactis genes were identified of which 292 were new.

  6. Y187 - Wikipedia

    en.wikipedia.org/wiki/Y187

    Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening.The strain has been sold commercially by Clontech [1] since at least 2000 and is used as a partner with strain AH109 in mating assays.

  7. Cloning vector - Wikipedia

    en.wikipedia.org/wiki/Cloning_vector

    Auxotrophic selection markers that allow an auxotrophic organism to grow in minimal growth medium may also be used; examples of these are LEU2 and URA3 which are used with their corresponding auxotrophic strains of yeast. [7] Another kind of selectable marker allows for the positive selection of plasmid with cloned gene.

  8. A woman bypassed multiple security checkpoints to get on a ...

    www.aol.com/woman-bypassed-multiple-security...

    Investigators are trying to determine how a woman got past multiple security checkpoints this week at New York’s JFK International Airport and boarded a plane to Paris, apparently hiding in the ...

  9. Candida albicans - Wikipedia

    en.wikipedia.org/wiki/Candida_albicans

    Next to the above-mentioned selection makers a few auxotrophic strains were generated to work with auxotrophic makers. The URA3 marker (URA3 blaster method) is an often-used strategy in uridine auxotrophic strains; however, studies have shown that differences in URA3 position in the genome can be involved in the pathogeny of C. albicans. [119]

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